John Wilkes Booth was an American stage actor who assassinated President Abraham Lincoln at Ford's Theatre in Washington, D.C., on April 14, 1865. He is ancestor of FBI Special Agent Seeley Booth.
In "The Parts in the Sum of the Whole" partway through the flashback, Booth stops Bones and tells her he has something to confess. Before he can spit it out, though, she counters with a typically blunt response, asking "What? Is it the fact that you're a direct descendant of John Wilkes Booth? I already know that." When Booth asks how she could possibly have inferred that fact, she reveals that it was the agent's bone structure that gave the connection away. He then asks her to keep the information a secret.
In "The Proof in the Pudding" Bones and Booth are having a conversation about Presidential assassination plots when Bones alludes to the sensitive subject that Booth is related to the most famous presidential assassin in history. After she brings it up, Booth responds, "You promised you would never mention that. You said that to me."
In "The Fact in the Fiction" Booth says if he could, he would go back in time and save President Lincoln from being shot.
In "The Secret in the Service", Aubrey is given a new partner when the Secret Service prevent Booth's involvement due to his relation to John Wilkes Booth.
- Being Seeley Booth's ancestor this is reason where believed to have Booth inherited marksmanship abilities. Though it should be noted that John Wilkes Booth was an actor not a soldier and shot Lincoln at point blank range, according to some sources even putting the barrel of the .44 caliber Derringer directly against his head. Another theory as to how this affected Booth's life is that it led to John Wilkes Booth's descendants wishing to redeem the bloodline for their ancestor's actions, which would explain Booth, his brother, father and grandfather all having history in service.